353 Necessary and sufficient conditions for keratinocytes to induce psoriatic dermatitis

The transcriptional activation of keratinocytes has essential roles for the specific types immune response skin. However, necessary and sufficient conditions in development psoriasis have never been identified. We previously demonstrated that mice lacking TRAF6 specifically are resistant to psoriatic inflammation induced by imiquimod. In addition, we found daily topical treatment with anisomycin, a p38 MAP kinase activator, on mouse ear skin is induce dermatitis an IL-17-dependent manner. signaling molecule between various receptors exogenous or endogenous mediators NF-κB pathways. Therefore, TRAF6-dependent, kinase-mediated expected generate psoriasis. To identify these conditions, selected differentially expressed genes (DEGs) shared two conditions: (A) Psoriasis overlap genesin vivo, (B) TRAF6-p38 vivo vitro. included fifteen genes, were imiquimod (84 genes) anisomycin (306 (518 4 times higher more lesional than nonlesional nine imiquimod-induced fully abrogated keratinocyte-specific knockout (80 genes), anisomycin-induced abolished inhibitor BIRB796 (282 2 primary-cultured after (124 genes). Only CXCL2 HBEGF, (B). These results suggest transcription may take part triggering inflammatory loops epithelial-immune microenvironment (EIME)